Molecular beacons for multiplex detection of four bacterial bioterrorism agents.
نویسندگان
چکیده
plasmon resonance (SPR). FEMS Immunol Med Microbiol 2000;29:283–8. 7. Asai R, Nakamura C, Ikebukuro K, Karube I, Miyake J. Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis. Biotechnol Lett 2002;24:1677–82. 8. Feriotto G, Borgatti M, Mischiati C, Bianchi N, Gambari R. Biosensor technology and surface plasmon resonance for real-time detection of genetically modified Roundup Ready soybean gene sequences. J Agric Food Chem 2002;50:955–62. 9. Nilsson P, Persson B, Larsson A, Uhlen M, Nygren PA. Detection of mutations in PCR products from clinical samples by surface plasmon resonance. J Mol Recognit 1997;10:7–17. 10. Feriotto G, Corradini R, Sforza S, Bianchi N, Mischiati C, Marchelli R, et al. Peptide nucleic acids and biosensor technology for real-time detection of the cystic fibrosis W1282X mutation by surface plasmon resonance. Lab Invest 2001;81:1415–27. 11. Feriotto G, Ferlini A, Ravani A, Calzolari E, Mischiati C, Bianchi N, et al. Biosensor technology for real-time detection of the cystic fibrosis W1282X mutation in CFTR. Hum Mutat 2001;18:70–81. 12. Riordan JR, Rommens JM, Kerem B, Alon N, Rozmahel R, Grzelczak Z, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA [Erratum in: Science 1989;245:1437]. Science 1989; 245:1066–73. 13. Poole JC, Andrews LG, Tollefsbol TO. Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT). Gene 2001;269:1–12. 14. Fivash M, Towler EM, Fisher RJ. BIAcore for macromolecular interaction. Curr Opin Biotechnol 1998;9:97–101. 15. Myszka DG. Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors. Curr Opin Biotechnol 1997;8:50–7. 16. Shastry BS. SNP alleles in human disease and evolution. J Hum Genet 2002;47:561–6.
منابع مشابه
تشخیص همزمان سه باکتری سالمونلا تیفی، باسیلوس آنتراسیس و یرسینیا پستیس با راه اندازی مولتی پلکس پیسیآر
Background and Objectives: Salmonella typhi, Bacillus anthracis, and Yersinia pestis are potent human pathogenic bacteria that cause typhoid fever, anthrax, and plague,respectively. The classic microbiological methods for detection and identification of these agents are laborious and time consuming.Therefore, developing an accurate method for rapid detection of these potent human pathogens is i...
متن کاملMultiplex detection of single-nucleotide variations using molecular beacons.
We demonstrate that single-nucleotide differences in a DNA sequence can be detected in homogeneous assays using molecular beacons. In this method, the region surrounding the site of a sequence variation is amplified in a polymerase chain reaction and the identity of the variant nucleotide is determined by observing which of four differently colored molecular beacons binds to the amplification p...
متن کاملMolecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.
A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.
متن کاملSemiautomated clone verification by real-time PCR using molecular beacons.
Conventional, high-throughput PCR analysis of common elements utilizing numerous primer sets and template DNA requires multiple rounds of PCR to ensure optimal conditions. Laborious gel electrophoresis and staining is then necessary to visualize amplification products. We propose novel multicolor molecular beacons, to establish a high-throughput, PCR-based sequence tagged site (STS) detection s...
متن کاملNew Methods for Identifying Microorganisms as potential bioterrorism agents with Emphasis on Chromatography-Mass Spectrophotometry (GC-MS): Narrative Review
Today, with the development of microbiology, biotechnology, and cellular and molecular genetics, human knowledge of microorganisms has increased and the possibility of making biological weapons with pervasive effects has increased. On the other hand, bioterrorist events and the construction of laboratories and sites for the production of biological weapons in many developed and developing count...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 50 6 شماره
صفحات -
تاریخ انتشار 2004